Generator

Part:BBa_K1343001:Design

Designed by: Ronen Ben Jehuda   Group: iGEM14_Technion-Israel   (2014-09-22)


P_ompC->NheI->RBS->luxI->double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 109
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part has been sequenced and has been checked with a detector strain which detects AHL and produce color. We know for sure that it produces AHL.


Source

P_ompC: BBa_R0082 RBS: BBa_B0030 luxI: BBa_C0061 but we removed the LVA tag (gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc) and added stop codon: "tag". Double terminator BBa_B0015


References